LOCUS       40924_12755        4194 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway conversion vector pCON DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Kimura T, Nakao A, Murata S, Kobayashi Y, Tanaka Y, Shibahara K, 
            Kawazu T, Nakagawa T.
  TITLE     Development of the gateway recycling cloning system for multiple 
            linking of expression cassettes in a defined order, and direction on
            gateway compatible binary vectors
  JOURNAL   Biosci. Biotechnol. Biochem. 77 (2), 430-434 (2013)
  PUBMED    23391940
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Kimura T, Shibahara K, Tanaka Y, Nakao A, Kawazu T, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 4194)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4194)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2013"; volume: "77"; issue: "2"; 
            pages: "430-434"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pDONR201.
FEATURES             Location/Qualifiers
     source          1..4194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     652..668
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    676..800
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        825..855
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     misc_feature    1127..1175
                     /label=rare cutter sites
                     /note="rare cutter sites"
     protein_bind    complement(1442..1566)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2067..2166)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2289..3095
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3241..3829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3964..3991)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4083..4169)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtatagaaaa
      121 gtaggctgga tgaatcgaat aacttcttct tcaaatgcgc aaacgaagaa atttcctcag
      181 tctcagccac agccgccgga gaaaaatgag aaaaaactga aaagggagag atttccggtg
      241 aaccctttga gagatttcag tacaagaagc tctggaagca gcagttgtag caatgagagt
      301 gcttctggtt caacttccgg tgaagcatcc aacggttgtc acaggttctt gctctctcat
      361 tctttctcat cgtcttcttc atcatcttct tcttcgttgg gagtttttcc tcgtagacca
      421 gtgagatcag tagctaaaaa ccccaaatct gctccggtag ttagtaaacc cttaattaga
      481 aagaaaccaa gctctcttga ggaggttaag ttgaaatcta ctcttactga gaaacctaat
      541 ttgctgaagt ctcagagatg caagacaaat cctgtatctg gaaagagacc aacttgtaag
      601 attaccatga aaccggagaa agtttcggtt ttgaagaaac aatcttctgt ttctagaact
      661 agtggatccc ccatcacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
      721 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
      781 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
      841 ccggctcgta taatgtgtgg attttgagtt aggatccggc gagattttca ggagctaagg
      901 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      961 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     1021 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     1081 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattgcga tcgcatttaa
     1141 atggccggcc cttaattaag gcgcgccgtt taaacgggcg acggatggtg atccccctgg
     1201 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg
     1261 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg
     1321 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
     1381 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgatcga
     1441 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     1501 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     1561 agtggtgata tcaagcttcg acaattattt tggactctca tgttctcaag ctgaaatcca
     1621 aaagaaacat gatgttggtt cagatttgga atcactgaca atgaagtttc agtcagccgg
     1681 tttgtcacca aggatccagg cttcatcatg ggagccgagc cgttcaagtt tcaacttcga
     1741 ctatttggcc acatcttctg attctattga tctctcacag tttcagagag gtttagttga
     1801 caggagttct tgtcatcctc atgtaacact ggataaagtg tcaaggacac atttgagagt
     1861 agagcaaaca aatagccaca tgcctgaaat gaaatcccaa cagattacag atactgaatt
     1921 tgatattcaa aatcacaaag aaagtgctgc ttctctggga aaaggaagag aattgcttcc
     1981 atgctctgct gcagagtcaa taagcactga tggaggtggt ctgatttgtt ctgaggattc
     2041 aaattggatg gcatgttaca agaattaccc aactttatta tacaaagttg gcattataag
     2101 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2161 tatttgccat ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     2221 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     2281 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     2341 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     2401 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     2461 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     2521 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     2581 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2641 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2701 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2761 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2821 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2881 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2941 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     3001 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     3061 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     3121 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     3181 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3241 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3301 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3361 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3421 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3481 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3541 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3601 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3661 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3721 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3781 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3841 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3901 gttatcccct gattctgtgg ataaccgtat taccgctagc caggaagagt ttgtagaaac
     3961 gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc
     4021 gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc
     4081 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     4141 tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgc
//
